The Effect of Pmsg Dose on Corpora Lutea and Embryo Numbers in Gilts

Superovulation in donors is an important factor influencing ovulation rate and embryo recovery. Crossbreed pubertal gilts (n=376) treated from year 2005 to 2009 were used as embryo donors. Experiment was evaluated in two parts: In the first part (I) gilts were divided into two groups with different synchronization schedules. In the first group (R+) 265 gilts were treated with Regumate Porcine (JANSSEN). In the second group (R-) were gilts (n=111) without Regumate diet. The number of ovulations was higher in R+ group (15.1 ± 9.3) compared to Rgroup (14.9 ± 9.3). There was no significant difference (p>0.05) between both groups. The number of recovered embryos in R+ group (8.75 ± 8.06) and Rgroup (8.81 ± 6.63) did not significantly differ (p>0.05) between groups. In the second part of experiment (II) gilts were divided into four groups with different superovlulation schedules regardless Regumate treatment. Gilts were synchronized and superovulated by intramuscular injection dose from 400 to 600 i.u. of PMSG group A (n= 21), dose from 650 to 750 i.u. of PMSG group B (n= 61), dose from 800 to 850 i.u. PMSG group C (n= 189) and dose from 900 to 1000 i.u. of PMSG group D (n= 104) followed 500 i.u. of HCG 72 hours later (both groups). There were significant differences in the number of corpora lutea between groups A-B (p<0.05), groups A-D (p<0.05), groups B-C (p<0.05) and groups C-D (p<0.05). The number of embryos was 9.9 ± 7.4 (group A), 6.1 ± 5.8 (group B), 8.3 ± 6.6 (group C) and 11.3 ± 9.0 (group D). There were significant differences between groups A-B (p<0.05), groups B-C (p<0.05), groups B-D (p<0.01) and groups C-D (p<0.01). ). The highest number of recovered embryos was in group D which shows a high number of ovulations too.